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Fig. 6 | BMC Genomics

Fig. 6

From: Identification of circRNA-associated ceRNA networks using longissimus thoracis of pigs of different breeds and growth stages

Fig. 6

Enhancement of C2C12 cell differentiation by circKANSL1L (A) Relative RNA levels of muscle cell differentiation marker genes in C2C12 cells following circKANSL1L overexpression or inhibition. GAPDH served as the reference gene. (B) Protein levels of MYOG and MyHC in C2C12 cells following circKANSL1L overexpression or inhibition (left panels). MYOG and MyHC band intensities were quantified by Image J and normalized against GAPDH (right panels). (C) Relative RNA levels of MyHC I and IIb in C2C12 cells following circKANSL1L overexpression or inhibition. (D) Protein levels of MyHC I and IIb in C2C12 cells following circKANSL1L overexpression or inhibition (left panels). MyHC I and IIb band intensities were quantified by Image J and normalized against GAPDH (right panels). Values are mean ± S.E.M. Data represent six independent assessment methods. Student’s t-test was used to compare expression levels or values among different groups. *P < 0.05; **P < 0.01

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BMC Genomics

ISSN: 1471-2164

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