Fig. 5

Chromosome FISH karyotype of the Ns-genome chromosomes using probes pSc200 (red), pTa71A-2 (green) and Oligo-44 (red) for P. juncea and Leymus species. The arrangement of chromosomes was based on the FISH patterns similarities with P. huashanica (ZY3156) chromosomes and chromosomes morphology including arm ratio and relative length. White arrows indicate the FISH signal variations within homologous chromosomes, red lines indicate the chromosomes which can’t be discriminated from chromosomes of P. huashanica using all the three probes. Scale bar: 10 μm