Table 3 RT-PCR primers and annealing temperatures for DE circRNAs
CircRNAs | Forward 5' → 3' | Reverse 5' → 3' | product length (bp) | Tm/°C |
|---|---|---|---|---|
circ_0018936 | AGTCCCTGAGTTACGCCTT | TGGCCTTACCGATGATGACA | 253 | 55 |
circ_0003667 | TCCTTCTCTGGGGTTCGAAA | TTGCATAAAACTCCTCCCGCTA | 136 | 57 |
circ_0006661 | ACAGGAGCCGATATTTAGAGACC | TGCTGCCATAGCGACTTGCC | 173 | 58 |
circ_0020271 | TAATCCGCTGAAGATCCCT | CTCATCACACTGTACCCAT | 205 | 54 |
circ_0018320 | CTCTAGAAGCATTGGAGCAC | GCAGATGAAGAAAATACGCTCA | 222 | 55 |
β-actin | TCCGTGACATCAAGGAGAAGC | CCGTGTTGGCGTAGAGGT | 267 | 52–65 |