Fig. 3

Expression characteristics of circUBE2Q2 in cells and tissues. A CircUBE2Q2 formed by the circularization of exons 4–9 of UBE2Q2 gene shearing, and the length of circUBE2Q2 was found to be 494 bp. The red arrow indicates the back splice site. B CircUBE2Q2 was examined by RT-qPCR after treatment with RNase R or mock in total RNAs samples derived from MuSCs. The circular junction of circUBE2Q2 was identified using divergent primers using Sanger sequencing. C The expression levels of circUBE2Q2 in different tissues of embryonic cattle. D The fluorescent in situ hybridization results showed that circUBE2Q2 was mainly located in the cytoplasm. Magnification 73x. E HE staining of muscle tissue of Guangxi cattle at 18 (C18) and 24 (C24) months of age. F The analysis of muscle fiber density, diameters, and area for Guangxi cattle. G, H The expression of circUBE2Q2, MyOD1, MyOG, and MyHC were measured by RT-qPCR of the fetus and at 18 and 24 month-old muscle (G), MuSCs, and SVFs (H). I-K The expression of circUBE2Q2, PPARG, and C/EBPα were measured by rRT-qPCR of the muscle tissue, intramuscular fat, subcutaneous fat (I and J), and adipogenically differentiated SVFs (K). Values are means ± SEMs for three individual animals. ∗ P < 0.05, ∗∗∗ P < 0.001. Scale bars = 200 μm