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Fig. 4 | BMC Genomics

Fig. 4

From: CircUBE2Q2 promotes differentiation of cattle muscle stem cells and is a potential regulatory molecule of skeletal muscle development

Fig. 4

Effect of circUBE2Q2 on proliferation and apoptosis of Guangxi cattle MuSCs. A and C) Cell proliferation was detected with 5-ethynyl-20-deoxyuridine (EdU)(A), the EdU-positive cell index statistics are shown (C). B and D Cattle MuSCs were transfected with circUBE2Q2, and the cell phase was analyzed (B) and counted (D) by flow cytometry. E Cell proliferation was detected with the cell counting kit-8 (CCK-8) assay. F The expression of the proliferation marker genes, PCNA and cyclin D1, were measured by RT-qPCR. G and H Cattle MuSCs were transfected with circUBE2Q2, and cell apoptosis was determined (G) and counted (H) by annexin V-FITC/PI binding followed by flow cytometry. I Cattle MuSCs were transfected with circUBE2Q2, and cell apoptosis was determined by Hoechst 33342/PI dual staining assays. J The mRNA expression of apoptosis marker genes (Bax and p21) by RT-qPCR. Values are means ± SEMs for three individual animals. ∗ P < 0.05, ∗*∗ P < 0.001. Scale bars = 200/400 μm

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