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Fig. 6 | BMC Genomics

Fig. 6

From: RNA-seq coupling two different methods of castration reveals new insights into androgen deficiency-caused degeneration of submaxillary gland in male Sprague Dawley rats

Fig. 6

Key candidate genes mediating androgen deprivation-caused degeneration of SMG. (A) Expression of receptors for reproductive hormones in SMG. (B) Number of putative androgen receptor (AR) binding site in promoter regions of common downregulated genes involved in epithelium development, anatomical structure morphogenesis, angiogenesis and cell apoptosis. De novo motif analysis was performed by Jaspar with 3 kb upstream and 500 downstream (promotor) sequence for the genes. 80% was used as the relative profile score threshold. Note common DEGs shown herein are the ones with fold change ≥ 1.5 at least in one pairwise comparison. Functional enrichment analysis was analyzed using DAVID (6.8). (C) qPCR validation of the key candidate genes mediating androgen deprivation-caused degeneration of SMG. Data are expressed as mean ± SEM; different superscripts indicate P < 0.05. B4galt1, beta-1,4-galactosyltransferase 1; Angptl4, angiopoietin-like 4; Ace, angiotensin I converting enzyme; Klf4, Kruppel like factor 4; Egf, epidermal growth factor; Tgfb2, transforming growth factor, beta 2; Wnt4, Wnt family member 4; Sox10, SRY-box transcription factor 10; Ccnd1, cyclin D1; Dnase2b, deoxyribonuclease 2 beta; Kcnip3, potassium voltage-gated channel interacting protein 3

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