Fig. 2

Verification of the target relationship between miR-148a-3p and DYNLL2. A Analysis of the correlation coefficient between DYNLL2 and target miRNA expression. B Binding free energy analysis of DYNLL2 and target miRNAs. C Conservation analysis of miR-148a-3p and DYNLL2-3’UTR binding sites in different species (chicken, human, cow, pig, and rat). D Site sequences of the wild-type vector for DYNLL2-3’UTR (DYNLL2-3’UTR-WT) and the mutant-type vector for DYNLL2-3’UTR (DYNLL2-3’UTR-MT) of the miR-148a-3p binding site. E The dual-luciferase reporter system assessed the binding of DYNLL2 and miR-148a-3p in DF-1 cells. F Expression of DYNLL2 and miR-148a-3p in chicken embryos from embryonic day 10 to 1 day old. r is the correlation coefficient between the expression of DYNLL2 and miR-148a-3p. G Changes in DYNLL2 after transfection with miR-148a-3p mimics in the pre-differentiation and differentiation period. All experiments were performed in triplicate, and the data are expressed as the mean ± S.E.M. (* p <0.05; ** p <0.01, *** p <0.001).