Fig. 6

Experimental verification of the circRNA225-gga-miR-1306-5p-HSPA8 regulatory interaction. A Schema of miR-1306-5p binding site in chicken circRNA225 and HSPA8 3’UTR sequence. B Luminescence was measured after co-transfecting the wild-type or mutant sequence of circRNA225 with miR-1306-5p mimics (or mimics negative control (NC)) in 293 T cells. C Luminescence was measured after co-transfecting the wild-type or mutant sequence of HSPA8 3’UTR with miR-1306-5p mimics (or mimics NC) in 293 T cells