Fig. 3

Representative examples of Von Kossa′s staining on the scales of juvenile O. mykiss collected at different time points during salinity acclimation, and histomorphometric analyses of the stained scales. Positive Von Kossa′s staining sites (which stains the presence of calcium phosphates a brown colour) were mainly located in the middle of scales (white arrows); and unmineralized areas were mainly at the scale margins (red arrows). (A): CG; (B): 7D; (C): 14D; (D): 21D; scale bar = 500 μm. (E): Histomorphometric analyses. Data were expressed as means ± standard deviations (\(\overline{\mathrm{x}}\) ± SD); N = 10 scales. Different letters represented significant difference (P-value < 0.05)