Fig. 3
Coding gene (mRNA) expression profiles of virgin alate and mated dealate queen brains. A Volcano plot analysis of coding gene expression variation. Blue and red points correspond to the significantly enriched mRNA (DEGs) in the virgin and mated queens respectively with the |log2(fold-changes)|≥ 1 and FDR < 0.01. (B) Hierarchical clustering of the 295 DEGs in the 8 independent biological replicates. For each biological replicate, the values were calculated by averaging four technical runs of RNA-seq libraries. Red represents the high expression levels and Green represents the low expression levels. (C) Volcano plot analysis of lncRNA expression variation. Blue and red points correspond to the significantly enriched lncRNA (DELs) in the virgin and mated queens respectively with the |log2(fold-changes)|≥ 1 and FDR < 0.01. (D) Hierarchical clustering of the 65 DELs in the 8 independent biological replicates. For each biological replicate, the values were calculated by averaging four technical runs of RNA-seq libraries. Red represents the high expression levels and Green represents the low expression levels