Fig. 5

Relationships between MSTRG.6523 lncRNA and protein-coding gene expression. A The co-regulation network of MSTRG.6523 lncRNA and other DEGs in the mated dealate samples. Solid lines and red color in DEGs denote the positive correlation and dash lines and blue color in DEGs denote negative correlation. (B) The expression levels of MSTRG.6523 lncRNA (x axis) and the protein-coding gene LOC105192919 encoded as hexamerin 1 (y axis) correlate in mated dealate queen brains. Each dot represents one biological sample (virgin, n = 16; mated, n = 16). Correlation coefficient (rho) and p-value from Spearman correlation of mated samples is indicated. (C) Triplex-forming oligonucleotide (TFO) motif within MSTRG.6523 lncRNA (green) identified using Triplexator software to target triplex sites (TTSs) in hexamerin 1 locus (blue and red) to form a RNA:DNA:DNA triplex. (D) Predicted triplex binding sites of hexamarin 1 locus (purple box) with MSTRG.6523 lncRNA and the AG-rich sequences are presented in the first intron of hexamarin 1 gene