Fig. 7

Nc909 lncRNA expression associated with DEGs. A  A co-regulation network of nc909 lncRNA with DEGs in the virgin and mated groups, respectively. Solid lines and red color in DEGs denote the positive correlation and dash lines and blue color in DEGs denote negative correlation. (B) The expression profile of nc909 and LOC105200275 (chymotrypsin inhibitor gene) shows a significantly positive correlation across both virgin and mated samples. Nc909 lncRNA expression has significantly negative correlations with LOC105201089 (C), LOC105195192 (D), and LOC105197201 (E) across the mated samples (orange) but no correlation across virgin samples (blue). (F) RPISeq predictions on the nc909 lncRNA and two annotated proteins of PRC2 core components in S. invicta. (G) The catRAPID outputs for the signal localization of nc909 lncRNA and two PRC2 core proteins binding propensity prediction with Global Score value, signal localization plot which denotes the localization of the signal along the RNA sequence colored according to the Global Score value, and a table with the information of the binding loci with a Z-score representing the interaction propensity in contrast to benchmark value of a pool of RNA-binding proteins. (H) Proposed model of the up-regulated nc909 lncRNA transcripts interacting with PRC2 to inhibit gene expression in the mated dealate queens brains