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Table 2 Comparison of the genome coverage and assigned clade for run116 samples on Nextclade

From: Comparison of SARS-CoV-2 sequencing using the ONT GridION and the Illumina MiSeq

 

Coverage (%)

Clade

Sample

GridION

MiSeq

GridION

MiSeq

K013400

72

92

20C

20A

K013408

57

86

20H (Beta, V2)

20C

K013410

70

90

20C

20A

K013411

76

93

20H (Beta, V2)

20A

K013415

63

91

20C

20A

K013417

63

94

20C

20A

K013418

62

94

20C

20A

K013423

63

91

20C

20A

K013425

60

93

20C

20A

K013426

57

89

20C

20A

K013429

64

95

20H (Beta, V2)

20C

K013432

65

93

20C

20A

K013433

50

94

20C

20A

K013434

68

94

20C

20A

K013437

35

92

20H (Beta, V2)

20C

K013445

65

91

20C

20A

K013447

92

98

20A

20D

K013449

49

94

20C

20A

K013450

68

97

20C

20A

K013452

68

94

20C

20A

K013454

56

90

20C

20A

K013462

51

92

20C

20A

K013465

60

94

20C

20A

K013467

50

92

20C

20A

K013470

72

89

20C

20H (Beta, V2)

K013476

69

91

20C

20H (Beta, V2)

Total

20A

1

20

20C

22

3

20D

0

1

20H (Beta, V2)

4

3

  1. The table above highlights the 27 samples which were sequenced on both the MiSeq and the GridION but were classified in different clades by Nexclade. Clades identified by the GridION include 20A (n = 1), 20C (n = 22), and 20H (Beta, V2) (n = 4). Clades identified by the MiSeq include 20A (n = 20), 20C (n = 3), 20D (n = 1), and 20H (Beta, V2) (n = 3). There was also an overall higher genomic coverage for sequences from the MiSeq when compared to the GridION.