Fig. 1

Experimental design showing the control treatment (A) and the three temperature switch treatments (B-D). Gonadal phenotypes were determined using RNA-seq and histology (bold), histology only (normal text) and for a subset of the control samples, RNA-seq only (italic text). Abbreviations: dpo, days post oviposition; dps, days post switch. A mortality rate of 80% occurred in the late development switch following a down switch at stage 12 to 36 °C to 28°