Table 2 Primer’s information used for qPCR of DoG family in P. edulis (Moso bamboo)
Accession number | Gene number | Sequence (5'-3') | Annealing temperature of PCR (℃) | Amplicon length (bp) |
|---|---|---|---|---|
PH02Gene44830.t1 | PeDOG6-F | GGAGAAGACAGACAAGAA | 76.5 | 90 |
PeDOG6-R | CGAATGAAACCACTCAAC | |||
PH02Gene37785.t1 | PeDOG9-F | AGCTGTCCAACCTTGAAG | 76 | 91 |
PeDOG9-R | CCTAAGACTGTCAATCCGT | |||
PH02Gene37919.t1 | PeDOG12-F | CAGACATTGATGAGAGGAA | 76.7 | 117 |
PeDOG12-R | AGTATTCTATGAAGCAGCAG | |||
PH02Gene39868.t1 | PeDOG14-F | GGGAATGGAAACGACAAC | 89.5 | 151 |
PeDOG14-R | CAAGAAGCTCACGACCTA | |||
PH02Gene16035.t1 | PeDOG18-F | GCACGATGGTTGGAAGAA | 75.1 | 95 |
PeDOG18-R | TAGAGGCAACACAACAACT | |||
PH02Gene31633.t1 | PeDOG19-F | TAGGCAACAGGGTGTGTA | 80.1 | 93 |
PeDOG19-R | ACAAACTCTACCTCATGCG | |||
PH02Gene24017.t3 | PeDOG21-F | AACAAGGTCCTGAGAAGA | 77.9 | 90 |
PeDOG21-R | TAGGTCGTTGATCTCTGT | |||
PH02Gene47106.t1 | PeDOG23-F | CACTACGAGAGGTTGTTC | 82.2 | 108 |
PeDOG23-R | GTGTAGAAGGAGACCTAG | |||
PH02Gene36596.t1 | PeDOG24-F | AGAAGGTCCTCAGAAGATT | 77.4 | 90 |
PeDOG24-R | TAGGTCGTTGATCTCTGTT | |||
Nucleotide tract-binding protein | NTB-F | TCTTGTTTGACACCGAAGAGGAG | 60 | 133 |
NTB-R | AATAGCTGTCCCTGGAGGAGTTT |