Comparative analysis of novel and common reference genes in adult tissues of the mussel Mytilus galloprovincialis

Table 1 Primer sequences

Gene		Primer sequence 5′-3′	Amplicon	Intron	Refs	E
*Ef1α-g*	F	TTTCTGGATGGCACGGAGAC	150 bp	No
*Ef1α-g*	R	CTGTGGGTCTTGATGGTGGG	150 bp	No
*Rpl34*	F	ACACAAAGAAGGCAGGTGCAG	136 bp	Yes		2.0
*Rpl34*	R	GACCCTCCATAAGCTCTGGTTAC	136 bp	Yes		2.0
*Rpl32*	F	CACTTGATGCCTGATGGTTTCC	143 bp	Yes		2.0
*Rpl32*	R	GCACGCTCAACGATTTCTTTCC	143 bp	Yes		2.0
*Rpl14*	F	CAATGCCAAGATCCCTCCGTAC	167 bp	Yes		2.0
*Rpl14*	R	AGCTTGCTTGGCCTTCATCAG	167 bp	Yes		2.0
*Act*	F	ATGCTCCAAGAGCCGTGTTTC	114 bp	Yes		1.9
*Act*	R	CTCTCTTGCTCTGGGCTTCATC	114 bp	Yes		1.9
*Cyp-A*	F	GGAGTTGAGAGCTGATGTTGTACC	100 bp	Yes		1.9
*Cyp-A*	R	TCGGTGGAATTTGCTGTCTTGG	100 bp	Yes		1.9
*Ef1α*	F	CCAGTGGCAAGACCTTATTCGAG	183 bp	Yes		2.0
*Ef1α*	R	TGGCTGGAGCAAAGGTAACAAC	183 bp	Yes		2.0
*18S*	F	AGAAACGGCTACCACATCCA	160 bp	Yes		2.0
*18S*	R	TGCCCTCCAATAGATCCTCG	160 bp	Yes		2.0
*28S*	F	AGTGCACTTTCCTCGGGTAG	123 bp	Yes		2.1
*28S*	R	GACGAGTCGACACTAGACGG	123 bp	Yes		2.1
*Gapdh*	F	AGGAATGGCCTTCAGGGTAC	114 bp	Yes	[19]	2.1
*Gapdh*	R	TCAGATGCTGCTTTAATGGCTG	114 bp	Yes	[19]	2.1
*Rps4*	F	TGGGTTATCGAGGGCGTAG	121 bp	Yes	[19]	1.9
*Rps4*	R	TCCCTTAGTTTGTGAGGACCTG	121 bp	Yes	[19]	1.9
*MGAL10A040115*	F	TGCCAACCACAGACTTCTCT	167 bp	Yes		1.9
*MGAL10A040115*	R	CTTTGCGTGGTTGGAAGTCA	167 bp	Yes		1.9
*MGAL10A054097*	F	AACAACCCCAGTACCACCTT	212 bp	Yes		1.9
*MGAL10A054097*	R	TCTTTAATGGCAAGCTGGGC	212 bp	Yes		1.9
*MGAL10A087091*	F	GACAACGGCCAAGGAATAGC	141 bp	Yes		2.0
*MGAL10A087091*	R	TTCGTTCCTGGACCACTCTG	141 bp	Yes		2.0
*MGAL10A005692*	F	GCAGGTGTTGTTGTCATGGT	156 bp	Yes		2.1
*MGAL10A005692*	R	AGAGAGAGCTTGGTTCGTGT	156 bp	Yes		2.1

F Forward, R Reverse, amplicon size in bp, presence of an intron within the amplified region and calculated reaction efficiencies (E) for RT-qPCR experiments

ISSN: 1471-2164