Fig. 5

Predicted Targets of human miRNAs across time following SARS-CoV-2 infection of lung epithelial cells identified by mirTarRnaSeq through interrelation analysis. Interrelation heatmap representing significant (mirTarRnaSeq adjusted p < 0.1 and miranda score 169, for miRNA and mRNA differential expression (DeSEq2 adjusted p < 0.1) and mirTarRnaSeq predictions) miRNAs and mRNAs at A 4 to 12 hrs, CHEK1 is also a predicted target of hsa-miR-155-3p based on TargetScan algorithm. B 12-24 hours C. 4 vs 24 hrs heatmap of all significant miRNA-mRNA interrelations predicted by both mirTarRnaSeq and miRanda. The heatmap units are the absolute difference between miRNA and mRNA fold changes (FC); gray color represents no significant interrelation identified by mirTarRnaSeq. For all heatmaps, columns represent the significantly differentially expressed miRNAs, vs the mRNA targets shown in rows identified by mirTarRnaSeq. D GO enrichment analysis for all the targeted genes in part C which are targets of human miRNAs in 4-24 hrs time point interval. E Comparison of mirTarRnaSeq miRNA-mRNA interrelations analysis with miRanda and Target scan filtering step predictions. F Gene ontology enrichment for all mRNAs predicted to be miRNA targets by mirTarRnaSeq (any miRanda score). G Heatmap of all miRNA-mRNA interrelations predicted by mirTarRnaSeq and miRanda in patient blood after COVID-19 infection (n = 8, 4 with COVID-19, 4 without COVID-19). The heatmap units are the absolute difference between miRNA and mRNA fold changes (FC); gray color represents no significant interrelation identified by mirTarRnaSeq