Fig. 4

SARS-CoV-2 whole genome sequencing using Sequence-Independent, Single-Primer Amplification (SISPA) coupled with Miseq Ilumina sequencing. Left panel (A) represents single SARS-CoV-2 virus genome assembly (the percentage of genome coverage after reference mapping and de novo assemblies). The virus was tenfold serially diluted, starting from viral load of 2.3 × 106 pfu/ml, mark as “0” on x-axis followed by 2.3 × 105 pfu/mL (mark as 1), 2.3 × 104 pfu/mL (mark as 2), etc. Right panel (B) represents genome assembly (the percentage of genome coverage after reference mapping and de novo assemblies) of two viruses, SARS-CoV-2 and influenza A(H1N1)pdm09 in mixed viruses single sample. SARS-CoV-2 virus was tenfold serially diluted, starting from viral load of 2.3 × 106 pfu/mL, mark as “0” on x-axis, that was spiked with constant amount of H1N1 virus (7.4 × 106 pfu/mL)