Fig. 3

Heatmap of the immunity genes for each sample and treatment detected as differentially expressed in the noble crayfish (a) Raw counts were transformed to transcripts per million (TPM), followed by standardisation with Z-score scaling (where Z score is calculated as follows: Z = s_i-μ/σ where s_i is the gene expression for a sample in TPM, μ is mean of the expression for each gene in TPM and σ is standard deviation of the expression for each gene in TPM). Therefore, the colours in the heatmap reflect the relative expression levels between samples per each gene, with higher expression in red and lower expression in blue. Hap A, haplogroup A; Hap B, haplogroup B, I and II, first and second sampling point, respectively (3 days and 21 days post-challenge), 1–5, identifying number of the crayfish (b) gene expression of the prophenoloxidase (proPO), CCAAT/enhancer-binding protein beta (EBP), and Krueppel like protein (KLP) in the marbled crayfish and the noble crayfish challenged with A. astaci. Expression values are shown in TPM