Fig. 2

WIZ regulates cohesin localization independently of G9a. A Overlap of SMC3 ChIP-seq peaks in WT and G9a KO cells. Heatmap shows the log2 ratio of ChIP-seq signal in G9a KO vs WT. Peaks identified in G9a KO cells but not WT cells which displayed a negative log2 ratio of ChIP-seq signal were removed from further analysis. B Overlap of shared (grey) and ectopic (green) SMC3 peaks with CTCF sites, DNA loop anchors, enhancers, and promoters. C Overlap of ectopic RAD21 peaks in Wiz^del cells with ectopic SMC3 peaks in G9a KO cells. Overlap is defined as within 2 kb. D Histogram showing the distance from an ectopic SMC3 peak in G9a KO cells to the nearest ectopic RAD21 peak in Wiz^del cells. Median (red solid line) and mean (red dashed line) of the distribution are indicated. E Heatmaps showing the log2 ratio of cohesin signal (perturbation / WT) at ectopic cohesin peaks identified in Wiz^del cells only (left heatmaps, sorted from highest to lowest signal in Wiz^del column), peaks identified as ectopic in both Wiz^del cells and G9a KO cells (center heatmaps, sorted from highest to lowest signal in Wiz^del column), and peaks identified only in G9a KO cells (right heatmaps, sorted from highest to lowest signal in G9a KO column)