Fig. 5
From: Chromosomal localization of cohesin is differentially regulated by WIZ, WAPL, and G9a
Ectopic cohesin peaks in Wizdel cells are enriched for various transcription factor motifs. A Table showing top 6 motifs identified by MEME-ChIP motif enrichment analysis for ectopic RAD21 peaks in Wizdel cells. RAD21 peaks shared between Wizdel and WT cells were used as background in the analysis. B Table showing top 6 motifs identified by MEME-ChIP motif enrichment analysis for ectopic RAD21 peaks in WAPL-AID cells. RAD21 peaks shared between WAPL-AID and WT cells were used as background in the analysis. C Table showing top 6 motifs identified by MEME-ChIP motif enrichment analysis for ectopic SMC3 peaks in G9a KO cells. SMC3 peaks shared between G9a KO and WT cells were used as background in the analysis. D Bar graph showing the percentage of SP1 peaks in WT mESCs which overlap all cohesin (RAD21 or SMC3) peaks in WT, all cohesin (RAD21 or SMC3) peaks in the perturbation condition (Wizdel, WAPL-AID, or G9a), and all ectopic cohesin (RAD21 or SMC3) peaks in the perturbation condition (Wizdel, WAPL-AID, or G9a). E SP1 ChIP-qPCR was performed in WT and Wizdel cells at three RAD21 ectopic sites, as well as a control site. Data are presented as mean ± SEM, n = 3 independent ChIP experiments performed with technical triplicates