Fig. 5
From: Identification of crucial circRNAs in skeletal muscle during chicken embryonic development
Validation of differentially expressed circRNAs. a agarose gel electrophoresis test for PCR products of divergent primers (DP) and convergent primers (CP) using cDNA (cD) and gDNA (gD). b Sanger sequencing confirmed the back-splicing junction of circRNAs; (c) RT-qPCR validation of five differentially expressed circRNAs in F14vsF20 and S14vsS20 respectively. NOTE: Original gels are presented in Supplementary Fig. S1; M: maker; DP: divergent primers; CP: convergent primers