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Fig. 10 | BMC Genomics

Fig. 10

From: Gene expression profiling of RIP2-knockdown in HD11 macrophages — elucidation of potential pathways (gene network) when challenged with avian pathogenic E.coli (APEC)

Fig. 10

New target genes of RIP2 upon APEC challenge. A The relative BID mRNA expression level in the group of wild type HD11 cells (WT), knockdown of RIP2 HD11 cells (shRIP2), avian pathogenic E. coli (APEC), and knockdown of RIP2 HD11 cells combined with APEC infection (APEC+shRIP2). (data are shown as mean ± SD; n = 4 independent experiments; different letters represent a significant difference, p < 0.001; same letters indicate no significance, p > 0.05). B The relative CASP9 mRNA expression level in group of wild type HD11 cells (WT), knockdown of RIP2 HD11 cells (shRIP2), avian pathogenic E. coli (APEC), and knockdown of RIP2 HD11 cells combined with APEC infection (APEC+shRIP2). (data are shown as mean ± SD; n = 4 independent experiments; different letters represent a significant difference, p < 0.001; same letters indicate no significance, p > 0.05). C The relative HSP90AB1 mRNA expression level in the group of wild type HD11 cells (WT), knockdown of RIP2 HD11 cells (shRIP2), avian pathogenic E. coli (APEC), and knockdown of RIP2 HD11 cells combined with APEC infection (APEC+shRIP2). (data are shown as mean ± SD; n = 4 independent experiments; different letters represent a significant difference, p < 0.001; same letters indicate no significance, p > 0.05). D The protein level of BID, CASP9, and HSP90AB1 in the group of wild type HD11 cells (WT), knockdown of RIP2 HD11 cells (shRIP2), avian pathogenic E. coli (APEC), and knockdown of RIP2 HD11 cells combined with APEC infection (APEC+shRIP2). E Image J software was used for BID gray-level analysis of western blot results (data are shown as mean ± SD; n = 3 independent experiments; different letters represent a significant difference, p < 0.05; same letters indicate no significance, p > 0.05). F Image J software was used for CASP9 gray-level analysis of western blot results (data are shown as mean ± SD; n = 3 independent experiments; different letters represent a significant difference, p < 0.05; same letters indicate no significance, p > 0.05). G Image J software was used for HSP90AB1 gray-level analysis of western blot results (data are shown as mean ± SD; n = 3 independent experiments; different letters represent a significant difference, p < 0.05; same letters indicate no significance, p > 0.05)

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