Table 2 The upregulated drought-responsive proteins in YW642 identified by MALDI-TOF/TOF-MS
Function classification/ spot ID | Accession No. | Protein Name (spot ID) | KOG number | Protein MW/PI | Protein Score C.I.% | Average %vol. ratio (YW642/control) at 15, 20, 25, 30, 45 DPA | p-value | Subcellular location prediction |
|---|---|---|---|---|---|---|---|---|
Stress/defense | ||||||||
  spot 6 | AAP80655 | Formate dehydrogenase | KOG0069 | 28.99/8.61 | 100 | 2, 0.46, 4.42, 1.32, 8.06 | 0.012 | Cyto |
  spot 29 | KAF6988077 | Glucose and ribitol dehydrogenase-like protein | KOG0725 | 31.88/6.54 | 100 | 1.88, 1.12, 1.89, 2.73, 0.36 | 0.021 | Cyto |
  spot 30 | KAF6988077 | Glucose and ribitol dehydrogenase homolog | KOG0725 | 31.88/6.54 | 100 | 2.59, 1.36, 2.53, 2.07, 2.78 | 0.003 | Cyto |
  spot 59 | P93692 | Serpin-Z2B | KOG2392 | 45.1/6.03 | 100 | 2.04, 1.49, 1.25, 4.19, 0.22 | 0.045 | Chlo |
  spot 61 | ACN59483 | Serpin 1 | KOG2392 | 43.1/5.44 | 100 | 1.6, 4.77, 2.13, 0.96, 3.82 | 0.024 | Mito |
  spot 65 | ADJ67792 | Peroxidase 1 | NA | 39.26/8.14 | 100 | 2.09, 1.7, 3.2, 0.57, 0 | 0.021 | Extr |
  spot 67 | AAL71854 | Dehydroascorbate reductase | KOG1422 | 23.46/5.88 | 100 | 2.13, 1.34, 3.13, 1.06, 0.46 | 0.034 | Cyto |
  spot 68 | CBM38934 | Hsp26 | KOG0710 | 27.42/5.82 | 100 | 2.12, 0.58, 0.11, 1.82, 0 | 0.001 | Cyto |
  spot 105 | ANW82830 | Formate dehydrogenase, mitochondrial | KOG0069 | 41.64/6.51 | 100 | 1.91, 1.23, 0.47, 2.08, 2.07 | 0.015 | Mito |
  spot 115 | ABO70341 | Pm3b-like disease resistance protein 15Q1 | KOG4658 | 59.2/6.27 | 100 | 0.97, 1.1, 0.78, 2.79, 1.06 | 0.014 | Cyto |
  spot 128 | AAF02296 | PR-4, partial | KOG4742 | 13.10/7.00 | 100 | 0, 1.1, 0.15, 3.97, 0.4 | 0.009 | Wall |
  spot 135 | BAA19099 | 2-Cys peroxiredoxin BAS1 | KOG0852 | 23.31/5.71 | 100 | 0, 2, 3.87, 2.04, 0.98 | 0.013 | Chloa |
  spot 137 | ACN59483 | Serpin 1 | KOG2392 | 43.10/5.44 | 100 | 2.06, 0.1, 0.41, 25.49, 0.63 | 0.012 | Mito |
  spot 145 | XP_044419905 | Glyoxalase I | KOG2943 | 32.56/5.57 | 100 | 1.66, 3.02, 2.3, 2.04, 0 | 0.011 | Cyto |
Energy metabolism | ||||||||
  spot 23 | XP_020185250 | Dihydrolipoyl dehydrogenase 1, mitochondrial | KOG1335 | 58.81/7.63 | 100 | 2.65, 1.14, 2.14, 0, 0 | 0.019 | Mito |
  spot 32 | XP_044405132 | Putative aconitate hydratase | KOG0452 | 93.9/5.66 | 100 | 5.24, 0.85, 8.77, 2.74, 0.94 | 0.026 | Cyto |
  spot 102 | CAC14917 | Triosephosphate-isomerase | KOG1643 | 26.35/5.38 | 100 | 1.99, 2.2, 1.19, 0.28, 0.73 | 0.048 | Cyto |
  spot 139 | EMS51931 | UTP--glucose-1-phosphate uridylyltransferase | KOG2638 | 50.86/5.76 | 100 | 2.28, 1.42, 1.81, 11.29, 0 | 0.036 | Cyto |
  spot 140 | CAA52636 | ATP synthase beta subunit | KOG1350 | 59.21/5.56 | 100 | 2.08, 0.19, 2.27, 1.1, 1.45 | 0.017n | Mito |
  spot 141 | AGH20062 | enolase | KOG2670 | 48.10/5.49 | 100 | 1.18, 1.95, 2.06, 0.49, 0 | 0.034 | Cyto |
Carbon metabolism | ||||||||
  spot 15 | CBH32516 | Alpha-glucan phosphorylase, H isozyme,expressed | KOG2099 | 93.8 /7.60 | 100 | 0.25, 1.38, 1, 2.8, 0.29 | 0.019 | Cyto |
  spot 16 | AAM13694 | Beta-D-glucan exohydrolase | NA | 67.71/6.86 | 90 | 0.15, 2, 0.95, 2.59, 0.21 | 0.03 | Chlo |
  spot 60 | SPT18490 | Endoglucanase | NA | 35.3/8.71 | 100 | 0.03, 1.37, 1.24, 2.04, 0.3 | 0.034 | Wall |
Starch metabolism | ||||||||
  spot 143 | AAY42618 | Dimeric alpha-amylase inhibitor | NA | 15.15/5.58 | 100 | 0, 0.86, 2.15, 4.22, 0 | 0.018 | Cyto |
Nitrogen metabolism | ||||||||
  spot 87 | XP_037485906 | Alanine aminotransferase 2 | KOG0258 | 56.45/6.2 | 100 | 2.28, 2.03, 1.24, 0.18, 0 | 0.033 | Cyto |
  spot 122 | CBM36829 | Pyruvate decarboxylase | KOG1184 | 65.83/5.43 | 100 | 2.71, 1.05, 2.59, 1.01, 0.71 | 0.016 | Cyto |
  spot 126 | CBM36829 | Pyruvate decarboxylase | KOG1184 | 65.83/5.43 | 100 | 2.03, 2.08, 0.03, 0, 0 | 0.034 | Cyto |
Protein metabolism/Folding | ||||||||
  spot 48 | AAL05264 | Betaine-aldehyde dehydrogenase | KOG2450 | 54.4/5.44 | 100 | 1.94, 0, 2.35, 0.04, 0 | 0.028 | Chlo |
  spot 131 | AAU82107 | 20S proteasome beta 5 subunit | KOG0175 | 29.94/5.54 | 100 | 1.56, 0.2, 2.32, 1.04, 0.57 | 0.026 | Cyto |
  spot 138 | EMS58427 | Elongation factor Tu | KOG0460 | 45.62/4.61 | 100 | 2.02, 1.36, 0.05, 0.48, 1.45 | 0.012 | Chlo |
  spot 144 | EMS61536 | 26S proteasome non-ATPase regulatory subunit 14 | KOG1555 | 32.93/5.88 | 100 | 2.13, 2.7, 3.67, 0, 0 | 0.034 | Cyto |
Transport | ||||||||
  spot 101 | ADK88900 | Outer membrane channel protein OEP16-2 | NA | 17.95/6.7 | 95 | 0.03, 1.9, 1.63, 2.64, 1.39 | 0.045 | Mito |
  spot 132 | EMS55440 | Kinesin-4 | KOG0239 | 108.78/6.91 | 100 | 2.09, 0.74, 0.94, 2.05, 0.48 | 0.025 | Cyto |
Storage protein | ||||||||
  spot 26 | ACB41346 | Triticin | NA | 62.76/6.43 | 100 | 2.55, 0.03, 1.49, 0, 0.03 | 0.048 | Nucl |
  spot 27 | AGO17690 | Gamma-gliadin | NA | 16.2/8.88 | 100 | 1.01, 2.19, 2.01, 1.95, 0 | 0.047 | Vacu |
  spot 39 | AFM30909 | Globulin-3A | NA | 66.3/8.48 | 100 | 42.79, 0.33, 2.03, 2.51, 0.36 | 0.037 | Vacu |
  spot 50 | EMS66832 | Globulin-1 S allele | NA | 56.9/9.1 | 100 | 3.33, 2.08, 1.11, 2.54, 0.13 | 0.036 | Vacu |
  spot 51 | EMS62417 | Globulin-1 S allele | NA | 55.59/7.77 | 100 | 0, 2.01, 3.58, 1, 0.95 | 0.047 | Vacu |
  spot 53 | ABS72144 | Alpha gliadin | NA | 33.47/8.19 | 100 | 0.19, 0.8, 2, 0.57, 5.42 | 0.033 | Vacu |
  spot 64 | AFM30909 | Globulin-3A | NA | 66.63/8.48 | 100 | 2.28, 0.8, 1.4, 0.47, 0.04 | 0.038 | Vacu |
  spot 79 | ACJ65513 | Globulin 3C | NA | 38.41/9.15 | 100 | 1.39, 0.07, 2.14, 2.15, 2 | 0.012 | Vacu |
  spot 111 | AFM30909 | Globulin-3A | NA | 66.63/8.48 | 100 | 1.19, 5.85, 0, 1.14, 2.12 | 0.028 | Vacu |
  spot 129 | ACJ65515 | Globulin 3B | NA | 56.90/7.36 | 100 | 1.34, 0.13, 3.55, 1.41, 0.48 | 0.023 | Vacu |
  spot 130 | ACJ65514 | Globulin 3 | NA | 66.31/7.78 | 100 | 0, 0.17, 3.79, 1.66, 3.7 | 0.047 | Vacu |
  spot 133 | EMS60011 | 12S seed storage globulin 1 | NA | 63.83/6.62 | 100 | 0.55, 1.04, 2.02, 3.25, 5.56 | 0.028 | Vacu |
  spot 134 | EMS62417 | Globulin-1 S allele | NA | 55.30/7.77 | 100 | 3.5, 1.06, 1.18, 2.07, 0.77 | 0.037 | Vacu |
  spot 136 | AAB27108 | Triticin precursor | NA | 56.92/9.37 | 100 | 1.86, 2.86, 2.2, 3.57, 0.85 | 0.039 | Vacu |
  spot 142 | AKW50839 | High molecular weight glutenin subunit | NA | 90.51/6.15 | 100 | 14.55, 0.4, 6.07, 1.38, 1.86 | 0.018 | Nucl |
  spot 146 | ACJ65514 | Globulin 3 | NA | 66.31/7.78 | 100 | 0.88, 0, 2.23, 4.7, 0 | 0.019 | Vacu |
Others | ||||||||
  spot 2 | KAF6997295 | BJ297754 Y | NA | 38.5/5.02 | 100 | 0, 2.43, 0.34, 1.82, 4.52 | 0.03 | Chlo |
  spot 20 | KAF6997295 | G356.108B02F010919 | NA | 36.4/5.13 | 100 | 2.74, 2.03, 1.75, 1.07, 0.95 | 0.034 | Nucl |
  spot 49 | EMS54324 | Hypothetical protein TRIUR3_03549 | KOG0710 | 16.82/6.19 | 100 | 0.62, 1.2, 0.11, 2.17, 0.98 | 0.021 | Nucl |
  spot 55 | EMS66582 | Hypothetical protein TRIUR3_24891 | NA | 22.69/8.42 | 100 | 0.21, 0.37, 1.4, 1.27, 2.01 | 0.034 | Nucl |
  spot 66 | KAF7049137 | G608.111J02F010910 | NA |  | 100 | 0.18, 1.14, 2.95, 0.58, 0.4 | 0.001 | Nucl |
  spot 123 | KAF6997295 | Hypothetical protein F775_14176 | NA | 45.64/7 | 100 | 1.05, 5.69, 4.4, 2.22, 1.31 | 0.018 | Nucl |