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Table 4 Primers used for the amplification and sequencing of the mitochondrial genome of P. tomentosa. LR next to the fragment number means that long-range PCR was used to obtain the amplicon. See Additional file 1 for the full list of sequencing primers

From: Inverted base composition skews and discontinuous mitochondrial genome architecture evolution in the Enoplea (Nematoda)

Fragment No

Gene or region

Primer name

Sequence (5’-3’)

Length (bp)

F1

16S

Eno 16SF

GTTTKTGACCTCGATGTTGN

171

  

Eno 16SR

CYTTTWGTTCCTTTCGTACT

 

F2 (LR)

16S-cox1

MX F1

AACGTCTGTTCGACGTAAGA

3395

  

MX R1

CTACATCCATACCTACGGTG

 

F3

cox1

Eno COX1F

GATTHTTNGGTCAYCCTGAAGT

614

  

Eno COX1R

ATACCGWCGNGGTATACCAT

 

F4 (LR)

cox1-tRNA-Thr

MX F2

GATTGCCATGAATGATAGGA

7150

  

MX R2

CAAAATCTATATTCTACTTAAAC

 

F5 (LR)

nadL-16S

MX F3

CAAAACCAATAATTCTGTGTG

3469

  

MX R3

TCTTACGTCGAACAGACGTT

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