Fig. 7

Flow cytometry analysis of Haliotis discus hannai (DD) and hybrid H. discus hannai ♀ × H. fulgens ♂ (DF) hemocytes during short-term hyposmotic stress: A Total hemocyte count (THC), B Hemocyte mortality, C Reactive oxygen species (ROS), and D Phagocytosis. “0 h”: control group; “3 h”, “12 h”, and “24 h”: exposure times at low salinity. One-way ANOVA, followed by Turkey’s HSD test, was used to analyze the differences between the sampling times for each species. Analysis of the differences between the species at a given sampling time was done by Welch’s t-test. Asterisks = significant difference between DD and DF at the given sampling time: “*”: P = 0.02; “**”: P = 0.002; “***”: P = 0.000. The bar indicates no significant difference between sampling times (P > 0.05) for the individual species