Fig. 1

Cellular model and targets selection. A Transdifferentiation of BLaER1 pre-B cells into macrophages is accompanied by a dynamic transcriptomic remodeling of the cells. BLaER1 lymphocytes transdifferentiate into functional macrophages in the presence of Interleukin 3 (IL-3) and Macrophage colony-stimulating factor (M-CSF) upon β-estradiol induced release of CEBPaER to the nucleus. B Flow cytometry analysis of cell surface markers at T0, T3 (3 days) and T6 (6 days) after induced transdifferentiation in the BLaER1-Cas9 cell line. During the process, BLaER1 cells progressively lose the CD19 (B-cell marker staining -X-axis-) and gain the Mac1 (macrophage marker staining -Y-axis-). C Merged k-means clustered expression profiles (color code) of peaking and upregulated genes during transdifferentiation: 16 initial clusters of lncRNA (n = 174) and 36 initial clusters of protein coding genes (n = 939). FPKM values were log10 transformed before the normalization to z-score. Each line shows the expression pattern of a gene along transdifferentiation. The color corresponds to the k-means cluster to which the gene belongs (see also Supplementary Fig. S1 and S2)