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Table 4 Description of the treatments. Content of essential (Met, Lys, Thr) and non-essential (Ala, Asp and Asn) amino acids in the controla and supplemented feedsb offered in double-choice (DC) tests (T1, T2 or T3)c performed in slow (SG)- and fast-growing (FG) chickens

From: Alanine-specific appetite in slow growing chickens is associated with impaired glucose transport and TCA cycle

Amino Acid

Type

T1: Control feed (%)a

T2: EAA Supplemented feed (%)c

T3: NEAA Supplemented feed (%)c

Methionine (Met)

EAA

0.428

0.740

–

Lysine (Lys)

EAA

1.626

2.813

–

Threonine (Thr)

EAA

1.161

2.009

–

Alanine (Ala)

NEAA

1.427

–

2.468

Aspartic acid (Asp)

NEAA

2.735

–

4.732

Asparagine (Asn)

NEAA

1.368

–

2.366

  1. Acronyms: EAA essential amino acids, NEAA non-essential amino acids
  2. aThe control feed was a standard commercial broiler feed (Darwalla Group, Esk, QLD, Australia). See Table S1 in Additional file 1
  3. bThe EAA or NEAA supplemented feeds consisted of the control diet supplemented with a mix of Met, Lys and Thr (EAA) or Ala, Asp and Asn (NEAA), respectively. All amino acids were supplemented to reach 73% excess of the control diet, the maximum level with no significant impact on feed intake (Baker and Han, 1994; Mack et al., 1999)
  4. cThe double-choice (DC) tests T1, T2 and T3 consisted of a control DC offering two identical control feeds (T1) or a DC between the control feed and either the EAA (T2), or the NEAA (T3) supplemented feeds