Fig. 2

The leptin receptor (LEPR) gene and protein structure and polymorphisms in sheep. A Schematic nucleotide sequence structure of the LEPR gene and sheep polymorphisms previously reported to be associated with reproductive phenotypes (dashed arrows) or identified in the present study (solid arrows). Arrows indicate the position within the gene and the type of base pair exchange. Numbers, when reported, indicate position within the cDNA sequence ENSOART00000011314.1. B LEPR protein structure based on the long form (1165 amino acids) and polymorphisms previously reported (dashed arrows) or identified in the present study (solid arrow). Arrows indicate the position within the protein and the type of amino acid exchange. NTD: N-terminal domain; CRH: cytokine receptor homology; Ig: immunoglobulin-like domain; FNIII: fibronectin type III; Leptin binds to its homodimer receptor through CRH2 and activates downstream effectors through Box 1 domain and phosphorylation (P) of Y-residues (Y986, Y1078, Y1141) (adapted from Berger et al. [22]). C Multiple alignment of the LEPR protein sequences from mouse (NP_666258.2), rat (NP_036728.1), human (NP_002294.2), pig (NP_001019758.1), cattle (NP_001012285.2) and sheep (W5PL31) species with Weblogo software [23]. Only the C-terminal end of the protein is represented. A red arrow indicates the P1019 position in sheep. Numbering on the X-axis resulted from the multiple alignment and not the ovine sequence