Fig. 1

Insulin induces widespread alterations in chromatin accessibility and transcription. Serum-starved S2 cells were treated with vehicle or insulin for 4 h, and nucleic acids were isolated and analyzed. A Principal component analysis of chromatin accessibility determined by ATAC-seq. B Principal component analysis of transcript expression by RNA-seq. C Proportions of each genomic feature type in all annotated chromatin peaks analyzed by ATAC-seq in S2 cells after treatment with insulin or vehicle. D Proportion of each annotated feature type in each biological replicate. The data represent the mean ± SD. None of the proportions were significantly different