Fig. 6

Cloned DNA from differentially-accessible chromatin regions can induce luciferase activity upon insulin application. DNA was cloned in front of a minimal promoter and luciferase gene, S2 cells were transfected for 18 h and then treated with insulin or vehicle for 4 h. A Candidate ATAC-seq peaks were selected by the largest log₂ fold change. B Chromatin peaks from promoters (1-2 kb from the TSS) were the feature that was most highly correlated with differential transcript expression. Peaks with the highest log₂ fold change from this correlation were cloned upstream of luciferase for functional validation. C Chromatin peaks with significantly different accessibility were selected from introns, a genomic feature known to contain regulatory regions. Data represent means ± standard error of three biological replicates. Differences were analyzed by Student’s t-test