Skip to main content
Fig. 3 | BMC Genomics

Fig. 3

From: Time-series transcriptomic screening of factors contributing to the cross-tolerance to UV radiation and anhydrobiosis in tardigrades

Fig. 3

g127777 localizes in the Golgi apparatus and enhances anti-oxidative stress response in HEK293 cells. a The g12777 protein was fused to GFP and transfected to HEK293 cells. Cells were transfected with CellLight Golgi-RFP and stained with DAPI. Yellow arrows indicate the co-localization of g12777 and Golgi-RFP. Scale bar 25 µm. The images have been enhanced for visualization. b Cell transfected with g12777-GFP were exposed to hydrogen peroxide for 30 min and subjected to MTT assay after 24 h incubation. Error bars indicate standard deviation. Only the full-length g12777-GFP show an increase in survival at around 0.2–0.3 mM. ANOVA + Tukey HSD (* FDR < 0.05). c Cells transfected with g12777-GFP were exposed to hydrogen peroxide for 30 min and subjected to MACS flow cytometry to detect SYTOX blue and AnnexinV-Alexa 657 fluorescence after 24 h incubation. Error bars indicate standard deviation. Only full-length g12777-GFP show an increase in survival. ANOVA + Tukey HSD (* FDR < 0.05). d Peroxidase activity of the recombinant protein lacking the N terminal 62aa region (g12777-Δ62). Peroxidase function was present only when manganese ions were present. Error bars indicate standard errors. ANOVA + Tukey HSD (* FDR < 0.05)

Back to article page