Hierarchical clustering of targets shows dynamic miRNA-induced regulation of biological processes across F. hepatica life stages. Heatmap of gene expression (TPM) of targets that are negatively correlated to the transcription of associated miRNAs. Hierarchical clustering of gene target expression performed using One Pearson correlation on the average TPM of each mRNA cross the sample types. Gene expression presented from a scale of the minimum expression (low expression - blue) versus the maximum expression (high expression - red) of the specific gene across the sample types. Each cluster was analysed for gene ontology (GO) enrichment using hypergeometric tests, and GO terms selected on adjusted P value <0.001. Order of GO terms for each cluster organised from most significant to least significant.