Fig. 1

Culturation and identification of rBMMSCs. A, The culturation of rBMMSCs. a, Passage 0; b, Passage 1; c, Passage 2; d, Passage 3. B, Identification of rBMMSCs by four cell surface markers. C, Staining identification on 14-day osteogenic differentiation of rBMMSCs. a, Alizarin red staining in control group; b, Alizarin red staining in osteo-inductive group; c, Von Kossa staining in control group; d, Von Kossa staining in osteo-inductive group. D, qRT-PCR validation on 14-day osteogenic differentiation of rBMMSCs. E, Oil Red O staining identification for 21-day adipogenic differentiation of rBMMSCs. a, A magnification of 50×; b, A magnification of 200×. Independent t test was used in qRT-PCR validation. P < 0.05 considered significant difference, and ns indicated that the difference is not statistically significant