Table 1 The primers of lncRNAs, miRNAs and mRNAs for qRT-PCR validation
Gene name | Primer (5’ to 3’) | Primer Sequence |
|---|---|---|
Tug1(rat) | Forward | TGCTGAAGTTGTTTGCCTGC |
Reverse | TCCTTGGTGGAATTGGGCAC | |
AABR07011996.1(rat) | Forward | CGCTCACATTGCTCCTTGTT |
Reverse | CAGGCTCTGCACAACTGTTT | |
AABR07060133.1(rat) | Forward | GCTAATGGGAGTCTTGCAGC |
Reverse | AAGTAGTTCTCTGCGGCCTT | |
Nedd4l(rat) | Forward | CGCCGTGCTGTGAAAGATACCC |
Reverse | GTGTGACTTTGTGTTGTGGTTTGGG | |
Sgk1(rat) | Forward | AAGGAAACGTCAGTGCTCG |
Reverse | GGAGTTGTTGGCAAGCTTCTG | |
Fzd4(rat) | Forward | GTGGATCCTCGTGCCTTTCA |
Reverse | GGCATGGATAGCAGGGGTTT | |
GAPDH(rat) | Forward | GGCACAGTCAAGGCTGAGAATG |
Reverse | ATGGTGGTGAAGACGCCAGTA | |
ALP(rat) | Forward | AACGTGGCCAAGAACATCATCA |
Reverse | TGTCCATCTCCAGCCGTGTC | |
Runx2(rat) | Forward | CCATAACGGTCTTCACAAATCCTT |
Reverse | CTGTCTGTGCCTTCTTGGTTC | |
OCN(rat) | Forward | GGACCCTCTCTCTGCTCACTCTG |
Reverse | ACCTTACTGCCCTCCTGCTTGG | |
rno-miR-129-5p(rat) | Forward | CAGCTTTTTGCGGTCTGGGCTTGC |
rno-miR-93-5p(rat) | Forward | GCGCAAAGTGCTGTTCGTGCAGGTAG |
rno-miR-322-5p(rat) | Forward | CGCGTGCGCAGCAGCAATTCATGTT |