Fig. 1

The schematic illustration of circRNA annotation tool. RNA sequencing data compatible with circRNA analysis from distinct subcellular compartments were retrieved from GEO/SRA databases (top-left corner). The coordinates of backsplicing junction were extracted by using CIRI2. The previously archived circRNA information were downloaded from circBase and circRNADb (top-right). Both sources of data were re-annotated by using gene information from Ensembl database. Each backsplicing junction was mapped to a selected representative reference transcripts according to conservation/number of exon/length of transcripts and alignment to exon junctions. CircRNAs will be displayed along with their reference transcript (bottom-left) and/or with the coding region from their parental genes (bottom-right)