Fig. 6

Evolutionary rate analysis of extracellular protein domains of the group XIV of CTLDcps. A Schematic representation of the group XIV extracellular protein domains. Each domain is color-coded as in B. B Ka/Ks ratio of each protein domain was calculated for all the gnathostome taxon using one representative species per order. Box plots showing the median and representing each domain are color-coded: CTLD (light blue), sushi-like domain/hydrophobic stretch (red), Pro/Ser/Thr rich (purple). EGF-like domains were dividend in different subgroups based on their sequence signatures and annotated as: EGF-like (yellow), EGF 1 (orange), EGF 2 (light green), cbEGF 2 (calcium-binding EGF, dark green), Tme5 EGF (Thrombomodulin-specific EGF, violet). The y-axis represents the non-synonymous (Ka) over synonymous (Ks) substitution ratio. Ka/Ks = 1 indicates neutral evolution. Ka/Ks ≤ 1 indicates purifying selection, Ka/Ks > 1 indicates positive selection. Pairwise comparison of each representative species per order (CD93 n = 71, Clec14A n = 55, CD248 n = 55 and Thrombomodulin n = 67). Black dots indicate outliers