Table 2 Protologue for Allorhizobium ampelinum sp. nov

Genus name

Allorhizobium

Specific epithet

ampelinum

Species status

sp. nov

Species etymology

am.pe.li'num. Gr. n. ampelos grapevine; Gr. adj. ampelinos and N.L. neut. adj. ampelinum of the vine

Designation of the type strain

S4

Strain collection numbers

DSM 112012 ^T, ATCC BAA-846 ^T

16S rRNA gene accession number

U28505.1

Genome accession number

GCF_000016285.1

Genome status

Complete

Genome size

6,320,946

GC mol %

57.47

Country of origin

Hungary

Region of origin

Orgovány, Bács-Kiskun county

Date of isolation

1981

Source of isolation

Aerial gall on two-year-old woody grapevine (Vitis vinifera cv. 'Izsaki Sarfeher')

Sampling date

1981

Number of strains in study

8

Source of isolation of non-type strains

Grapevine

Growth medium, incubation conditions

used for standard cultivation

Yeast mannitol agar (YMA) at 28ºC

Conditions of preservation

-80 °C

Gram stain

Negative

Cell shape

Rod

Colony morphology

Colonies on YMA are white to cream colored, circular, convex and glistening

Positive tests with BIOLOG

pH 6, D-Mannose, D-Galactose, 1% Sodium Lactate, Pectin, Rifamycin SV, Tetrazolium Blue, Potassium Tellurite

Negative tests with BIOLOG

pH5, N-Acetyl Neuraminic Acid, 4% NaCl, 8% NaCl, 3-Methyl Glucose, Inosine, Fusidic Acid, Troleandomycin, D-Serine, Minocycline, Guanidine HCl, Niaproof 4, p-Hydroxy-Phenylacetic Acid, Lithium Chloride, γ-Amino-Butryric Acid, α-Hydroxy-Butyric Acid, α-Keto-Butyric Acid, Propionic Acid, Sodium Butyrate

Positive tests with API

URE, ESC, PNG, GLU (assimilation), ARA, MNE, MAN, MLT, OX

Negative tests with API

NO3, TRP, GLU (fermentation), ADH, GEL, CAP, ADI, PAC

Variable tests with API

NAG, MAL, GNT, CIT

Commercial kits used

BIOLOG GEN3, API 20NE

Oxidase^a

Positive

Positive tests^a

Growth at 35 °C, growth in nutrient broth supplemented with 2% NaCl, citrate utilization, production of acid from sucrose, production of alkali from tartrate

Negative tests^a

Production of 3-ketolactose from lactose, acid-clearing on PDA with CaCO₃, production of reddish-brown pellicle at the surface of ferric ammonium citrate broth, motility at pH 7.0, acid from d-( +)-melezitose, acid production from 4-hydroxyphenylacetate

Known pathogenicity

Plant pathogenic