Skip to main content
Fig. 7 | BMC Genomics

Fig. 7

From: Genome-wide alternative splicing profile in the posterior kidney of brown trout (Salmo trutta) during proliferative kidney disease

Fig. 7

PCR Validation of selected DEAS gene. cDNA from control (C1 to C6) and infected (I1 to I6) samples were used for PCR and the amplified PCR product was visualized using agarose gel electrophoresis (The complete images of agarose gel electrophoresis are provided in the Supplementary File S6). In the transcript structure, the boxes (green, red, and purple) indicates exons, the straight black lines represent introns, the splice junctions are represented by flexed lines (blue and red), and the position of primers is illustrated by red arrows. The position of the transcripts and their splice sites in the brown trout genome is proved in the Table. A Alternative 3′ splice site is represented by ENSSTUG00000027241 - prkcbp1l, (B) Alternative 5′ splice site is represented by ENSSTUG00000006981 - baz2ba, (C) Skipped exon is represented by ENSSTUG00000001109 - rap1gds1 and ENSSTUG00000012954 - pik3ap1, (D) Retention intron is represented by ENSSTUG00000037971 - rabgef1, and (E) Mutually exclusive exon is represented by ENSSTUG00000040153 mef2d

Back to article page