Fig. 3

Differential expression profiles of 5-GEC positive versus 5-GEC negative subgroup. A-C Differential expression profiles of 5-GEC positive aggressive T-ALL in adult patients (A and B) and children (C) of the training dataset. The volcano plots (left panels) and heatmaps (right panels) illustrate the transcriptomic profile of 5-GEC positive versus negative T-ALL in all adult patients of the training set (A, n = 54), in adult patients with MRD negative status (B, n = 25) or in children (C, n = 55). The p-values used in volcano plots were calculated with the Wilcoxon statistical test. The differentially expressed genes used for the heatmaps were selected with a p-value < 0.05 and abs (ratio) > 1.5. In all heatmaps the genes and samples were clustered using Euclidian-based distance hierarchical clustering with Ward’s linkage; D Correlation plots comparing gene expression signatures. Left panel: correlation plot comparing the gene expression signature of 5-GEC positive versus negative patients (x axis) with the signature of MRD positive versus negative patients (y axis) in all T-ALL patients of the training cohort (n = 109). Right panel: correlation plot comparing the gene expression signatures of 5-GEC positive versus negative patients considering adult T-ALL patients (n = 54, y axis) or considering adult T-ALL patients with MRD negative status only (n = 25, x axis)