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Fig. 1 | BMC Genomics

Fig. 1

From: Manipulating base quality scores enables variant calling from bisulfite sequencing alignments using conventional bayesian approaches

Fig. 1

An overview of the double-masking procedure. The central sequence represents the reference genome, with example alignments (+FW and -FW) adjacent to each originating strand. Black, emboldened nucleotides potentially arise from bisulfite treatment. Blue colouring indicates 5mC/5hmC. Red colouring represents in silico nucleotide manipulation, and corresponding base quality manipulations are indicated with an exclamation mark. In example (1) the variant caller is informed only by the -FW alignment, and in (2) only by the +FW alignment. As there is no equivalent Watson(+) alignment in (3) it is impossible to determine whether the apparent G >A polymorphism arises from bisulfite or a natural mutation

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BMC Genomics

ISSN: 1471-2164

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