Fig. 4

 A SNV-rich region on FM chromosome 5. A Genomic distribution of distinct SNVs between FM and PC. A red arrowhead indicates the region most enriched with distinct SNVs. B Composition of alleles at the SNV sites in the FM SNV-rich region. SNV sites were classified as homozygous (homo) for reference (Ref) or alterative (Alt) alleles or heterozygous (hetero) for two alleles. C Alleles at the selected SNV sites in the FM SNV-rich region. For each diallelic site, reference or alternative alleles in a homozygous or heterozygous state are shown as a color composite. Asterisks mark the sites within SNP clusters. D Distribution of the variant allele ratio (VAR) at heterozygous sites in the FM SNV-rich region. The VAR was calculated as the read count of the variant allele divided by the total read count at each heterozygous site. The number of heterozygous sites analyzed in each plot is indicated in parentheses. E Selective validation of loss of heterozygosity (LOH) and alteration of heterozygosity (AOH) in the FM SNV-rich region using Sanger sequencing from genomic DNA