Fig. 6
From: Accurate recombination estimation from pooled genotyping and sequencing: a case study on barley
Pool sequencing (nPGMps) to estimate recombination rates (RR) in genomic windows of 50 MB. A.i Correlation plot of the RR from pooled genotyping (RRnPGM, y-axis) compared to the RR from simulated pool sequencing (RRnPGMps, x-axis) at 100 reads coverage for 42,077 variant loci at population sizes of 50 and 100. A.ii Similar to A.i, but comparing the RRnPGMps to RRconsensus. The number of variants in the genomic windows is illustrated by color, while the chromosomes are differentiated by shape. A larger scale of cM/MB was observed for nPGMps than nPGM and the consensus map. B The average RR across 20 simulated replicated samples at given sequencing depth (x-axis) and the associated genomic window (y-axis). The samples are differentiated by color. B.i Correlation coefficient between RRnPGM and RRnPGMps. B.ii Correlation coefficient between RRnPGMps and RRconsensus. C Illustrating the effect of different genotyping depths on the correlation coefficient of RR on genotyping (C.i) and pool sequencing level (C.ii). C.i Comparing RRnPGM between samples with 42,077 (y-axis) and 10,000 (x-axis) variants genotyping depths; C.ii Similar to C.i on RRnPGMps