Fig. 9
ROS accumulation in terms of two stress markers i.e. superoxide radical (O2•−), hydrogen peroxide (H2O2) was visualized using (A) nitroblue tetrazolium (NBT) and (B) 3,3- diaminobenzidine (DAB) staining, respectively, 10-days-old wild type (WT), 35 S::At5g03350 overexpression (OE), and At5g03350: mutant (SALK_036814) seedlings (M) of Arabidopsis thaliana following 0, 2, 6 and 12 h of treatments with six abiotic stresses, cold, high light, oxidative (MV), UV-B, wound, and ozone. At least three independent experiments were performed for each analysis and five seedlings of each genotype were examined. Bar = 5 mm