Fig. 3
Plasmidic content analysis from genome sequence of the CI133 and CI172 strains. A Scale reconstruction of the virulence plasmid identified in the CI133 and CI172 strains, by homology of the sequences obtained with pCP301 and using the DNAMAN program. Red arrows indicate virulence genes identified in each strain; Green arrows indicate elements required for plasmid stability, black arrows indicate insertion sequences and the blue box indicates the origin of replication. The direction of the arrows indicates the orientation of each element with respect to the pCP301 sequence. The node or contigs where each element is found in each strain is indicated on each element. B Strain specific cluster of S. flexneri 2 CI172 strain showing the Colicin related genes and their probable plasmidic origin. C DNA plasmidic profile obtained from virulent CI133 and CI172 strains: 1-λDNA-HindIII molecular marker used to estimate the size of each plasmidic band, the size of each band is in the left indicated; 2- CI133 strain and 3- CI172 strain. The analysis was carried out by agarose gel electrophoresis and stained with ethidium bromide. DNAch: chromosomal DNA; p-I, pII and p-III putative plasmids identified by PlasmidFinder software