Fig. 2

ZNF143 OE significant inhibition of BRL-3A cell proliferation and cell cycle progression. A qRT-PCR analyses showing ZNF143 mRNA level after OE For each qRT-PCR experiment, three biological replicates and three technical replicates were analyzed. β-actin was used as an internal reference gene. B Western blot analyses showing ZNF143 protein level after OE. Two biological replicates and three technical replicates were analyzed. C CCK-8 assay analyses showing cell proliferation 24 h, 48 h, and 72 h after ZNF143 OE. For each CCK-8 assay, three biological replicates were analyzed. D GO (RNA-seq) enrichment analyses showing cell biological pathways enriched 72 h after OE. The data are presented as mean ± SD. P values: *P < 0.05; **P < 0.01 using two-tailed Student t test (A, B, C); FDR ≤ 0.05 (D)