Fig. 5

A Homology searches of putative Zisupton transposons in P. pacificus identified most closely related sequences in other metazoan phyla as well as green algae and fungi. The phylogenetic tree was reconstructed from a protein alignment of the Kyakuja-Dileera-Zisupton transposase domain (PF18758). The distinct grouping of the two orthologous families in P. pacificus from a homolog in the nematode Plectus sambesii suggests independent horizontal gene transfers. B Orthology analysis across ten diplogastrid genomes elucidates the recent evolutionary history of Zisupton activity. The plot shows the numbers of orthologs for both families across the schematic phylogeny. While the orthologous family OG0000357 seems to have arisen only recently, both families showed rapid expansions in the P. pacificus lineage. C Syntenic analysis identified evidence of recent transposon activity in P. pacificus. The plot shows gene structures of syntenic regions in P. pacificus, P. exspectatus, and P. arcanus. Orthologous genes are indicated by identical colors. While synteny is conserved between the two outgroup species, the P. pacificus genome shows a specific insertion of a putative Zisupton transposon