Fig. 3

The expression pattern of 6 differentially expressed genes (DEGs) in the leaves of foxtail millet varieties with different levels of leaf tannin content. Transcription levels were verified by qRT-PCR with SiACTIN gene as an internal control. The X axis represents different foxtail millet varieties, and the Y axis (left side) indicates the relative expression level of selected genes determined by qRT-PCR (blue columns). The transcript level of genes in variety 56229 was set as 1.0, and error bars represent standard error (n = 3). The Y axis (right side) depicts the expression level of genes in RNA-seq data (evaluated by FPKM, red lines)