Table 8 Differentially expressed ASFV genes in high and medium dose groups. TND = transcript not detected
ORF (Gene) | Differential expression in High dose | Differential expression in medium dose | Differential expression in Low dose | Description/Function | Reference | |||
|---|---|---|---|---|---|---|---|---|
log2 Fold Change | Padj | log2 Fold Change | padj | log2 Fold Change | padj | |||
A151R | 2.68 | 2.74E-02 | 2.99 | 1.60E-02 | TND | TND | Inhibits Absent in Melanoma 2 (AIM2) inflammasome activation. | [95] |
A240L (TK) | 2.10 | 4.80E-02 | 2.38 | 4.63E-02 | TND | TND | Thymidylate kinase Involved viral in DNA synthesis | [96] |
B646L (p72) | 2.34 | 4.80E-02 | 2.25 | 4.76E-02 | TND | TND | Encodes the variable major capsid protein, p72, an immunogenic protein in natural infections. Involved in virion assembly and entry. Late transcription gene. | |
C84L | −2.74 | 7.99E-03 | −2.82 | 8.18E-03 | TND | TND | Uncharacterised protein | [99] |
CP204L (p30) | 2.23 | 4.80E-02 | TND | TND | TND | TND | Plays a role in virus cell tropism, and is essential for effective virus entry and replication in macrophages. | [10] |
E184L (j12L) | 2.13 | 4.80E-02 | 2.25 | 4.76E-02 | TND | TND | Uncharacterised protein | Â |
EP402R (CD2v) | 2.08 | 4.81E-02 | 2.28 | 4.76E-02 | TND | TND | Similar to host CD2 protein, needed for binding red blood cells to infected cells and extracellular virus particles; responsible for heamadsorption in infected cells; glycoprotein inserted into external virus envelope | [100] |
I177L (k14L) | 2.13 | 4.80E-02 | 2.61 | 3.54E-02 | TND | TND | Uncharacterised protein | [100] |
I196L (k15L) | 2.15 | 4.80E-02 | 2.37 | 4.72E-02 | TND | TND | Uncharacterised protein | [100] |
I215L (k13L) | TND | TND | 1.51 | 4.76E-02 | TND | TND | Ubiquitin-conjugating enzyme | [100] |
I73R (k10R) | 2.24 | 4.80E-02 | 2.59 | 3.54E-02 | TND | TND | Uncharacterised protein | [100] |
K78R (p10) | 2.05 | 4.97E-02 | TND | TND | TND | TND | DNA-binding protein p10 involved in morphogenesis | [100] |
MGF 100-2 L | 2.82 | 2.45E-02 | 3.22 | 1.18E-02 | TND | TND | Modulate host cell functions | [91] |
MGF 100-3 L | 2.79 | 2.45E-02 | 3.15 | 1.18E-02 | TND | TND | ||
MGF 360-15R | 2.55 | 3.57E-02 | 2.78 | 2.79E-02 | TND | TND | ||
MGF 360-22R | 2.21 | 4.80E-02 | 2.48 | 3.85E-02 | TND | TND | ||
MGF 360-4 L | 2.08 | 4.81E-02 | 2.50 | 3.85E-02 | TND | TND | ||
NP1450L (g2L) | 2.26 | 4.80E-02 | 2.28 | 4.76E-02 | TND | TND | RNA polymerase subunit 1 is involved in transcription | [98] |