Fig. 4
From: Poly(a) selection introduces bias and undue noise in direct RNA-sequencing
Omission of poly(A) selection captures shorter-tailed RNA species. A Scatter plot comparing mean tail lengths called by Nanopolish PolyA for each gene between selected and unselected libraries for Replicate 1. Dashed black line indicates the diagonal where tail length in both libraries is equal. Genes of interest shown in subfigure 4B highlighted in red. B Violin plots showing distributions of poly(A) tail lengths called by Nanopolish PolyA for genes highlighted in subfigure 4A. Red horizontal lines indicate the mean tail length. Kernel density estimation completed using default settings of Plotly python package (version = 5.3.1)